Early Diverging Fungus Mucor circinelloides Lacks Centromeric Histone CENP-A and Displays a Mosaic of Point and Regional Centromeres

Mucor circinelloides Growth and Differentiation in the Fungus A Subunit of Protein Kinase A Regulates

CENP-A, a conserved centromeric histone within kinetochore chromatin

Studies in several organisms have shown that eukaryotic centromere regions contain proteins involved in chromosome inheritance functions such as centromere structure, heterochromatin assembly and sister chromatid cohesion. Mutation, inhibition, knockout and reciprocal epistasis experiments have revealed functions for many centromere region proteins and their order in the centromere assembly pat...

A subunit of protein kinase a regulates growth and differentiation in the fungus Mucor circinelloides.

The cyclic AMP (cAMP)-dependent protein kinase A (PKA) signaling pathway plays a role in regulating development, growth, and virulence in a number of fungi. To determine whether PKA plays a similar function in zygomycete fungi, a mutant of Mucor circinelloides was generated that lacks pkaR1, one of the regulatory subunits of PKA. The mutant showed a reduction in growth and alterations in germin...

Biphasic incorporation of centromeric histone CENP-A in fission yeast.

CENP-A is a centromere-specific histone H3 variant that is essential for kinetochore formation. Here, we report that the fission yeast Schizosaccharomyces pombe has at least two distinct CENP-A deposition phases across the cell cycle: S and G2. The S phase deposition requires Ams2 GATA factor, which promotes histone gene activation. In Delta ams2, CENP-A fails to retain during S, but it reaccum...

Double-strand DNA breaks recruit the centromeric histone CENP-A.

The histone H3 variant CENP-A is required for epigenetic specification of centromere identity through a loading mechanism independent of DNA sequence. Using multiphoton absorption and DNA cleavage at unique sites by I-SceI endonuclease, we demonstrate that CENP-A is rapidly recruited to double-strand breaks in DNA, along with three components (CENP-N, CENP-T, and CENP-U) associated with CENP-A ...